FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_WT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_WT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9253367
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG120560.13028771040854642No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA94200.10180078235306134No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT93810.10137931414586712TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA22650.031.83174736
ACTCCAG24250.030.4533123
ACTCGAT23850.029.93665137
AGTCACA26250.028.39972128
CATTACT27100.028.15455433
CACACGT26850.028.02564412
ACATTAC28950.026.47628232
GCACACG29600.025.89487611
CTCCAGT32800.023.04855324
CCAGTCA33400.022.63450826
ATTACTC34100.022.06711234
CAGTCAC34500.021.91282827
AGCACAC37700.020.88827710
TCGTATG36900.020.67719345
CTCGTAT35000.020.4996944
CGTATGC37450.019.53241346
GTCACAT40300.018.75901829
ACACGTC44850.018.41676513
ACGTCTG42300.017.95500615
CACATTA42600.017.91052431
Produced by FastQC (version 0.11.5)