FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_spoVG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_spoVG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158291
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT196480.3809013489157553TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA31100.055.25677536
ACTCGAT31700.052.0027237
TTACTCG34400.050.05772435
GAACTCC37850.045.6799421
GTATGCC38950.043.6710147
CTCCAGT40000.042.87464524
TCGTATG39650.042.2821245
ATTACTC41100.041.9826234
AGTCACA42150.041.85018528
ACATTAC41900.041.84929332
CTCGTAT38300.041.1223744
TCCAGTC41800.041.11209525
CCAGTCA42450.040.8948326
GCACACG42950.040.82620611
TCGATCT37750.040.79436539
CACGTCT43050.040.6500714
GTCACAT43300.040.496229
CACATTA43050.040.4061731
CGTATGC42000.040.2496746
CACACGT44200.039.67161612
Produced by FastQC (version 0.11.5)