FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_spoVG_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_spoVG_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7994541
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG164310.20552774699635662TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT131390.16434964809111619TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG38950.056.07122843
ATCGGGG52650.048.52744342
ACTCCGG60600.048.2832632
ACTCCAG62600.047.29976323
GTCACTC62500.047.09544429
CACTCCG62450.046.85293231
AGTCACT63450.046.6661228
TCACTCC64700.045.49405730
GCACACG66400.044.54013411
CAGTCAC67150.044.25115227
CACACGT66800.044.11624512
CCAGTCA67900.043.453126
CTCCAGT69900.042.209824
CTCCGGA70050.042.11941533
TGAACTC70300.042.06920620
GAACTCC71350.041.5482121
AGCACAC72750.040.7967710
TCCGGAG73650.040.25071334
TCCAGTC74050.040.12782325
CGGGGGT24900.039.91920544
Produced by FastQC (version 0.11.5)