FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_spoVG_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_spoVG_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8017067
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT504370.629120350372524TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG71000.051.217741
AGTCACC79600.048.8938928
ACTCCAG81250.048.2886623
GTATGCC78300.047.69419547
CACACGT85150.046.3235812
GCACACG86000.046.0285211
TCGTATG80850.045.4972945
ACCGCTC85300.045.38046632
TGAACTC86300.045.2604620
CGTATGC82700.045.11433446
GAACTCC86850.044.97383521
CCAGTCA87100.044.72392326
CAGTCAC87300.044.58136727
ACACGTC88950.044.3052613
AGCACAC91000.043.576410
TCCAGTC90500.043.31440425
CTCCAGT90850.043.0704824
CACCGCT92150.042.31094431
CACGTCT92600.042.18092314
ATTATCT88850.041.47952739
Produced by FastQC (version 0.11.5)