FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_spo0A_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_spo0A_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7511579
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT96610.1286147692782037TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG24900.040.76245528
CCAGTCA34400.029.91235426
ACTCCAG34850.029.32525323
TATCTCG24500.029.28528641
CAGTCAC35650.028.96170827
GAACTCC35900.028.9550121
ATTCGTA36050.028.05784436
GTCACGA36850.027.92360729
CACACGT38350.027.3791812
GCACACG39300.026.62828611
CTCCAGT39600.025.80770324
TCCAGTC41100.025.03612925
ACACGTC43350.024.30183613
TGAACTC43200.024.0621520
ACGAATT44000.023.78374332
AGCACAC45100.023.43661910
GTATGCC29600.023.4118247
CGTATCT34550.023.40052439
CACGTCT45300.022.94668414
TCGTATC43900.022.40286338
Produced by FastQC (version 0.11.5)