FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_spo0A_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_spo0A_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197425
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT221740.24108921790609875TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA39400.048.32406636
ACTCGAT39800.046.3434437
TTACTCG44450.043.22762335
GAACTCC46150.042.39365421
GTATGCC50800.036.7218347
ATTACTC55100.035.0629334
GCACACG56700.034.93768711
GTCACAT55950.034.84302529
CTCCAGT56000.034.62442424
ACATTAC56250.034.4705332
TCGTATG55700.033.6170345
CCAGTCA58500.033.264426
CACACGT60100.032.90294312
CACATTA59750.032.62706831
AGTCACA60400.032.62362728
TGAACTC59650.032.56441520
CATTACT61450.031.6674933
TCCAGTC60900.031.66613625
CTCGTAT56750.031.63823544
ACTCCAG61800.031.31824723
Produced by FastQC (version 0.11.5)