FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_spo0A_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_spo0A_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8452542
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG99560.11778705151657336TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG28800.044.4785243
GAACTCC39550.042.56575421
GTCACTC43600.038.2907329
AGTCACT47050.035.9293428
TGAACTC47450.035.40517820
CGGGGGT17150.035.10153244
TCCGGAG48350.034.81852334
GCACACG49750.034.33115811
CACGTCT51400.033.70573814
CACACGT50750.033.65468212
TCACTCC50450.033.64668730
ACTCCAG51100.033.0817123
ATCGGGG46400.032.8874542
AGCACAC52300.032.8580310
TCCAGTC51250.032.7800125
ACACGTC52650.032.639613
CTCCAGT51700.032.63008524
CTCCGGA51400.032.61625733
CACTCCG51900.032.36947331
ACGTCTG53100.032.2970815
Produced by FastQC (version 0.11.5)