FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_79T0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_79T0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778114
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT108150.15955765866434232TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC24000.035.29099328
TATCTCG26850.030.24173241
ACTCCAG28750.030.06899323
CCAGTCA29350.029.93128426
GAACTCC31300.028.06655921
CACACGT31300.028.06655912
GTATGCC28850.027.65978447
CAGTCAC33550.026.18429827
GCACACG33100.026.0115911
CTCCAGT33250.025.99950424
TCCAGTC34150.025.8267425
ACCGCTC33350.025.50176232
TGAACTC35650.024.9364120
TCGTATG32600.024.907545
ACACGTC37900.023.91775113
AACTCCA37950.023.05621322
CACCGCT38900.022.94300731
AGCACAC39400.022.91834410
CTCATTA37450.022.61639236
CGTATGC36550.022.5029946
Produced by FastQC (version 0.11.5)