FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_79T0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_79T0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8975476
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT110160.12273443770558798TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG32250.042.6505728
GCACACG40300.034.21788411
CCAGTCA41300.033.4741126
CACACGT41000.033.37758312
ACTCCAG41650.033.10878423
GAACTCC42600.032.12396621
GTCACGA44100.031.42813529
ATTCGTA44250.030.29336236
CTCCAGT46350.029.90249424
TATCTCG33400.029.86485541
TGAACTC48550.028.18704220
GAATTCG48200.027.8834334
CAGTCAC49800.027.69037827
AGCACAC52100.027.0053810
AACTCCA51300.026.81249222
TCCAGTC52100.026.80384825
GTATGCC36800.026.34474247
ACACGTC53850.025.93278713
ACGTCTG54000.025.53668215
CGTATCT42200.024.96410839
Produced by FastQC (version 0.11.5)