FastQCFastQC Report
Thu 27 Sep 2018
H3WNHAFXY_n01_3_79T0_0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WNHAFXY_n01_3_79T0_0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10625274
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT182380.17164733822393663TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA35750.041.70559336
ACTCGAT36000.040.54098537
AGTCACA44550.034.48892228
ATTACTC46250.032.76701734
ACTCCAG47850.032.32980723
GTATGCC46100.032.1144347
TTACTCG47000.031.9462735
ACATTAC48450.031.8570532
GAACTCC48700.031.62164321
CACACGT50900.030.18613412
GCACACG50950.030.15651111
CCAGTCA51200.029.80432726
CAGTCAC52550.029.5714827
TCGTATG52450.028.56005545
CATTACT54200.028.21908433
TGAACTC55500.027.4319720
CGTATGC55200.027.39084246
GTCACAT56900.027.06457129
TCGATCT52100.026.73659339
ACACGTC58700.026.29440113
Produced by FastQC (version 0.11.5)