FastQCFastQC Report
Sat 18 Jun 2022
H3WMNBGXM_n01_GD_eRNA_R24_K562_A375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WMNBGXM_n01_GD_eRNA_R24_K562_A375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25102859
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC461308818.37674346177063No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA424741216.920032893464445No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT347066213.825763830327057No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG340777713.575254515830249No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT320065512.750161246573546No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG289203011.520719612056936No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC1247440.4969314451393763No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTATCAAC416610.16596117597601134No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAA373920.14895514490998815No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGGT291940.11629751017603214No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGG279640.11139766988294043No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACT278460.11092760390360318No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)