FastQCFastQC Report
Sat 18 Jun 2022
H3WMNBGXM_n01_GD_eRNA_R02_A375_Rep0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WMNBGXM_n01_GD_eRNA_R02_A375_Rep0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21618173
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG379377217.548994542693315No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT348214516.10748974948068No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT340177915.735737705494355No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC339746115.715763769676558No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG250325911.579419777980313No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA239468611.077189547886402No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGG327340.1514189011254559No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGGT269320.12458037041335546No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACT249560.11543991252174733No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTATCAAC246310.11393654773694335No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGT225480.10430113590079976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)