FastQCFastQC Report
Sat 18 Jun 2022
H3WMNBGXM_n01_GD_R21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WMNBGXM_n01_GD_R21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19015809
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT887966946.69624626541001No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT761328840.03662426352726No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT689730.3627139923418457No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT605760.3185559972757404No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTATCAACTGGT526300.27676971303193043No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTATCAACT514180.2703960688709063No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG373270.19629456732553424No Hit
TAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGTC371350.19528488112180764No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGTACCCCTACCAACTG321800.16922761477042603No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGACTACGACCTGAAT314160.16520990508476394No Hit
CGATCATGATCGTCTTGTGAAAGGACGAAACACCGTACCCCTACCAACTG309520.16276983009242468No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACTACGACCTGAATGCT281590.14808205109758937No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGTC273750.14395916576570578No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGTC226230.11896943222347259No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG210140.11050805148495128No Hit
CGATATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG207510.1091249917371383No Hit
TAAGTAGAGTCTTGTGGAAGGACGAAACACCGTACCCCTACCAACTGGTC203750.10714768958817371No Hit
TAAGTAGAGTCTTGTGAAAGGACGAAACACCGTACCCCTACCAACTGGTC200060.10520719891538666No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)