FastQCFastQC Report
Sat 18 Jun 2022
H3WMNBGXM_n01_GD_R20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WMNBGXM_n01_GD_R20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163519039
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA2488316815.21729099692177No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC2114682212.932330161260305No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT2001945812.242891177950233No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG1855214211.34555469103509No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA1691020110.341426358309262No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC157458219.629350255660443No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT135077168.260638077747021No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG106690166.524632278446793No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGCCCCTTGACCTT11436680.699409687700036No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTTATAGAACCTCC6341810.3878331256582299No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC3371450.20618088392752848No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1800660.11011928708803138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)