FastQCFastQC Report
Sat 18 Jun 2022
H3WMNBGXM_n01_Alex2_R14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WMNBGXM_n01_Alex2_R14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10331514
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG436019542.20286591103685No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG342491333.15015592100054No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG8621068.344430448431856No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGG6962776.739351076715377No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTG159260.15414972094119023No Hit
GATCGCGCGGTTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG149060.14427701496605433No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTG140630.13611751385130968No Hit
GATCGCGCGGTTCTTGTGGAAGGACGAAACACCGAACCCCTACCAACTGG140350.1358464983931687No Hit
ATACACGATCTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT124580.12058252062572823No Hit
GATCGCGCGGTTCTTGTGAAAGGACGAAACACCGAACCCCTACCAACTGG116980.1132263867619015No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGG111860.10827067552732349No Hit
ATACACGATCTCTTGTGGAAGGACGAAACACCGAACCCCTACCAACTGGT103760.100430585488245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)