FastQCFastQC Report
Mon 30 Nov 2015
H3WLMAFXX_n01_sv99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WLMAFXX_n01_sv99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17595480
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13500.026.33312842
TCGTATG14100.026.17000643
TCTCGTA14450.025.69213341
CGTATGC15800.023.6394644
CGCCAAT17700.023.13593933
TATCTCG17000.022.6322439
ATCTCGT18950.020.0660940
ACGCCAA21550.019.52509732
GTCACGC21550.019.1073429
CGTCTGA21300.019.01328716
ACGTCTG23600.017.06496215
AGTCACG26800.015.95200228
TCACGCC26250.015.94345830
ACACGTC26300.015.74078613
CACGCCA31100.014.03589931
CACGTCT29450.013.52235214
ATCGGAA33700.012.4185482
GATCGGA34350.012.250311
GTATGCC32300.011.84204645
GCCAATA36850.011.60092934
Produced by FastQC (version 0.11.2)