FastQCFastQC Report
Mon 30 Nov 2015
H3WLMAFXX_n01_sv98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WLMAFXX_n01_sv98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31442786
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1052990.33489080770387203TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG136900.041.25367443
CTCGTAT136700.040.85328742
CGTATGC145600.040.2099444
TCTCGTA141650.038.9809541
ATCTCGT144150.037.5869440
GTATGCC161200.036.48510745
CGTCTGA175200.035.49500716
ACACGTC181900.034.89285713
GATCTCG160250.034.44238739
ACGTCTG180950.034.441715
CACGTCT190750.032.719614
AGTGATC178000.032.1190936
CACACGT199000.031.8835212
ATCGGAA202950.031.796682
GATCGGA207250.030.9446471
GTGATCT178300.030.91784137
GTCACAC219800.028.87785729
TGATCTC192050.028.66887338
TCGGAAG230350.028.1026633
CGGAAGA232350.027.7639244
Produced by FastQC (version 0.11.2)