FastQCFastQC Report
Mon 30 Nov 2015
H3WLMAFXX_n01_sv96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WLMAFXX_n01_sv96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40626436
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2755570.6782701785605807TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG339100.042.1210843
CTCGTAT337950.042.01141442
TCTCGTA339500.041.8260841
CGTATGC346550.041.72803544
CGATGTA360300.041.40241634
CACCGAT369000.041.1474431
ACCGATG369500.041.1101332
TATCTCG348850.041.0853339
CCGATGT366250.040.8355533
ATCTCGT350800.040.491640
GTCACCG384500.039.99186729
TCACCGA385750.039.8565930
CGTCTGA385000.039.69757516
ACACGTC394650.039.4396613
GATCGGA401150.039.176511
ACGTCTG391500.039.03283315
ATCGGAA404800.038.8985022
CACGTCT400050.038.2042814
TCGGAAG417400.037.6700023
CGGAAGA419450.037.5341724
Produced by FastQC (version 0.11.2)