FastQCFastQC Report
Mon 30 Nov 2015
H3WLMAFXX_n01_sv100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WLMAFXX_n01_sv100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33313414
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2777140.8336401666908111TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG331900.042.8786343
CTCGTAT329850.042.81769642
CGTATGC338850.042.78928444
TCTCGTA331850.042.6546341
CGTCTGA373450.042.1428616
GATCGGA385650.041.906191
ACGTCTG377600.041.82275815
ACACGTC386200.041.77119413
ATCGGAA390200.041.6049042
ATCTCGT339300.041.5655440
CATCTCG351850.041.099839
TCGGAAG399300.040.6232263
CACGTCT390400.040.54955314
CGGAAGA403100.040.363084
GTATGCC366250.039.5806745
CACACGT407450.039.55972712
GCACACG413950.039.335211
GATCATC400350.037.19870436
GAACTCC449400.035.47074521
ACCAGAT450550.035.13284332
Produced by FastQC (version 0.11.2)