FastQCFastQC Report
Wed 26 Sep 2018
H3WJ3AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WJ3AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11199740
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG268450.094.247491
TCGCATC286000.094.179777
GTATCTC247000.094.11146
ACTGTAT247100.094.092533
TATCTCT248300.093.8892067
GCATCTT287250.093.8762369
ATGGTCT284200.093.840336
CTGTATC247900.093.808044
AATGGTC284400.093.707545
ACAATGG286250.093.516663
ATACAGG317550.093.494747
CGCATCT288450.093.379848
CGATAGG271350.093.19156
AACAATG287750.093.1290362
CGATACA320650.092.695825
CAATGGT287700.092.583174
TCGATAG274050.092.342675
GATACAG321500.092.303056
AGGTCGC336350.080.36374
TAGGTCG338400.079.315543
Produced by FastQC (version 0.11.5)