FastQCFastQC Report
Wed 26 Sep 2018
H3WJ3AFXY_n01_MYCScreens2_5_BC_bc23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WJ3AFXY_n01_MYCScreens2_5_BC_bc23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18043245
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3391750.094.9400561
GTCGCAT3479800.094.701196
GGTCGCA3478450.094.69975
TCGATAC3914000.094.689754
TAACAAT3748100.094.664461
TCGCATC3480450.094.663037
TAGGTCG3478350.094.635783
AGGTCGC3480400.094.5784154
ATAGGTC3483600.094.512772
TCGATAG3414050.094.421525
TACTGTA3265750.094.378952
CTGTATC3265600.094.33454
ACTGTAT3270250.094.223873
GCATCTT3497000.094.223179
TGTATCT3273700.094.138825
AACAATG3776000.094.118492
GTATCTC3274000.094.088136
AATGGTC3774500.094.040615
ATACTGT3276500.094.039931
CGCATCT3504100.094.030918
Produced by FastQC (version 0.11.5)