FastQCFastQC Report
Wed 26 Sep 2018
H3WJ3AFXY_n01_MYCScreens2_5_BC_bc22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WJ3AFXY_n01_MYCScreens2_5_BC_bc22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19905306
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3726050.094.9472351
GTCGCAT3803600.094.687146
GGTCGCA3802500.094.673315
TAACAAT4135100.094.664641
TCGCATC3804650.094.654777
TCGATAC4277100.094.6209264
TAGGTCG3803950.094.6027453
AGGTCGC3807750.094.494134
TCGATAG3750900.094.473935
ATAGGTC3812600.094.435932
TACTGTA3698850.094.427782
CTGTATC3701450.094.347674
ACTGTAT3702700.094.3047563
GCATCTT3823050.094.220329
AACAATG4160000.094.179862
GTATCTC3707500.094.1783456
TGTATCT3710250.094.1482245
TATCTCT3710150.094.112367
ATACTGT3710150.094.051871
AATGGTC4159400.094.010945
Produced by FastQC (version 0.11.5)