FastQCFastQC Report
Wed 26 Sep 2018
H3WJ3AFXY_n01_MYCScreens2_5_BC_bc19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WJ3AFXY_n01_MYCScreens2_5_BC_bc19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22736087
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4547500.094.933121
GTCGCAT4292450.094.6680456
GGTCGCA4289950.094.6600955
TAACAAT4618450.094.654551
TCGATAC4851750.094.6447144
TCGCATC4294900.094.632847
TAGGTCG4291750.094.590733
AGGTCGC4294250.094.5265964
ATAGGTC4298450.094.447712
TCGATAG4579500.094.434265
TACTGTA4119700.094.402172
CTGTATC4120550.094.372114
ACTGTAT4122650.094.32313
GCATCTT4313250.094.179589
TGTATCT4131750.094.133545
GTATCTC4129500.094.128476
AACAATG4649150.094.125112
TATCTCT4133650.094.063847
ATACTGT4132600.094.0526351
CGCATCT4321500.094.040468
Produced by FastQC (version 0.11.5)