FastQCFastQC Report
Wed 26 Sep 2018
H3WJ3AFXY_n01_MYCScreens2_5_BC_bc18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WJ3AFXY_n01_MYCScreens2_5_BC_bc18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20682109
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3493300.094.941821
GTCGCAT4210800.094.694986
TAACAAT4244350.094.694891
GGTCGCA4209550.094.677965
TCGCATC4211600.094.655577
TCGATAC4712600.094.6470644
TAGGTCG4214400.094.590653
AGGTCGC4214900.094.5330054
ATAGGTC4220500.094.4843142
TACTGTA3730350.094.414942
TCGATAG3521250.094.36965
CTGTATC3732250.094.353914
ACTGTAT3734500.094.29733
GCATCTT4231200.094.175579
TGTATCT3742350.094.152585
GTATCTC3741300.094.152346
AACAATG4276950.094.116792
CGCATCT4238050.094.0793848
TATCTCT3747850.094.048627
ATACTGT3742800.094.04671
Produced by FastQC (version 0.11.5)