FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_wt0_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_wt0_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5121067
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG968201.8906216224079866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG71050.13874061792200726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG68140.13305820837727764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT7400.036.89035837
TCGGTGG6700.036.56814251
GGAAAAA312900.033.1943270
GGGAAAA322550.030.6817869
GGGGAAA320650.030.29469368
ACTCTAG6400.030.0812825
GGGGGAA324250.029.67765667
GATAGGG9650.029.37701836
TACTCTA7900.027.9143224
GGATAGG9800.027.85979535
TAGGATA10400.026.25249933
GTGGTCG10050.026.12061354
CTCTAGT8000.024.9398756
GTAGGAT12750.024.43369932
GGTCGCC10950.023.97324656
GGTGGTC10450.023.78100653
GGGGGGA419650.023.1728766
CGGTGGT11400.021.79946952
GTAGGGA13200.020.9438519
TGGTCGC13150.020.76101355
Produced by FastQC (version 0.11.2)