FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_wt-1_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_wt-1_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653563
Sequences flagged as poor quality0
Sequence length76
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8655613.243711776829473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG101181.5481292545630643No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG94751.4497454721274001No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84271.2893936774266597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG69581.0646257514577784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG68491.0479479407494No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA66991.0249968250956678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG66541.0181114903995483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG49300.7543266678193227No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG45250.6923586555542465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG44380.679047008475082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG40390.617997040836155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG38770.5932098359301246No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC37190.5690346607748603Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG33530.5130339385797543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT32820.5021704105036545No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA32760.5012523658775053No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA32170.4922249270537041No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT31480.4816674138529874No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA29390.4496888593754542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG28300.43301104866707574No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT27180.41587421564562255No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA25300.38710881735961183No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT24940.3816005496027162No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG23690.3624746198912729No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG19770.30249570431618683No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA19570.2994355555623559No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC17760.27174120934018603No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.2708231647140368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGG16910.2587355771364046No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGCTATC16510.25261527962874275No Hit
GCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCT16390.2507791903764442No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA16220.24817806393568792No Hit
ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGAGATCGGAA15490.23700852098420505ABI Solid3 Adapter B (100% over 41bp)
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC15340.23471340941883184No Hit
CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGATAGATCGGAA14190.21711755408430405ABI Solid3 Adapter B (100% over 41bp)
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC14110.21589349458277168No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGG13040.1995216987497762No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT12990.1987566615613185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTGGGGG12590.19263636405365664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTGGGGG12410.18988223017520883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGG12320.1885051632359849No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT11990.18345591779216386No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGG11750.17978373928756675No Hit
GCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCA11010.16846118889839234No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA10990.16815517402300925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGGGGG10760.1646360029561037No Hit
GATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATAGGCT10580.16188186907765587No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG10490.16050480213843193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGG10400.15912773519920803No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT10290.15744465338460104No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA10070.15407848975538702No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC9860.15086533356386453No Hit
CTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAA9820.15025330381309834No Hit
CTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA9540.14596909555773505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGG9480.1450510509315858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG9480.1450510509315858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGTGG9380.14352097655467033No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT9370.14336796911697877No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG9340.14290894680390415No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT9240.14137887242698868No Hit
TAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATT9190.14061383523853094No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCT9090.1390837608616155No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG8930.13663564185855073No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGTGG8880.13587060467009301No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG8770.13418752285548602No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT8760.13403451541779446No Hit
CCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTA8540.13066835178858044No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTGGGGGG8530.1305153443508889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGG8400.1285262476608988No Hit
TTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTAC8400.1285262476608988No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTGGGG8320.12730218815936642No Hit
CTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGAT8310.1271491807216749No Hit
AGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAAC8230.12592512122014252No Hit
TAGACACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAA8150.12470106171861015No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA8040.12301797990400312No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT7520.11506159314404274No Hit
GCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACAGT7450.1139905410802019No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGG7160.10955332538714706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGTGGGGGG7100.1086352807609978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGG7100.1086352807609978No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG7050.10787024357254006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGTGG7000.10710520638408233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGG6950.10634016919562461No Hit
GGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG6950.10634016919562461No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC6930.10603415432024152No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGTGGGG6900.10557513200716688No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAG6760.10343302787948522No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT6550.10021987168796274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTG2250.070.015395
CCGTATG207.920439E-570.01002561
TCCCGTA150.002223210470.01002553
CAATATT150.002223885470.0046633
TATTCCC150.00222591169.98859436
GCCCATA2200.069.9832411
CCATAGA2200.069.9832413
ATCACCG2300.068.50381
ACCGACT2300.068.493314
CGACTGC2300.068.488076
TGCCCAT2250.068.433310
CCCATAG2250.068.4280612
GCGTAGT2350.067.0257533
CTGCCCA2300.066.9558649
GACTGCC2400.065.63447
CACCGAC2400.065.63443
CATAGAG2350.065.5162314
CATTAAA3900.064.62958567
TTGTACT13400.064.538991
CCACTAC1900.064.4977341
Produced by FastQC (version 0.11.2)