FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472199
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111930.3223605559473982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT3500.020.0078751
TAGGGAG5050.019.4071532
GGGAGTT5800.016.89760434
GGGTAAT7950.015.8552971
TGTGCTA4650.015.80333918
GGGGGGA21200.015.68787866
ATAGGGA6750.015.55630131
TAGTCGA5050.015.2458419
CTAACGT11700.014.9631551
GGGGAAA20500.013.83272168
CTAGGTA5200.013.4654812
CTATAAC6500.013.45927511
GGGGGAA21850.013.45873767
TGTGGTA3405.0733433E-813.38010324
CTCAACT9800.013.2194881
AGGATCC1900.001891808412.8964713
GCTAGAT4651.200533E-1012.8007371
CTAGGGC9950.012.66337764
AATAGGG8350.012.57545430
TAGCCTC4751.72804E-1012.5249328
Produced by FastQC (version 0.11.2)