FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5166092
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108100.20924908034932402No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG60900.11788407949374499No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA59640.11544509853870198No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA52790.102185559219619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAG11000.029.26674520
CAACTAG11250.028.92742218
CTAGAGT11150.028.8730221
CAATAGA11500.028.6072839
CCAATAG11800.027.5833838
ATTAGAG13300.027.1068087
AGAGGTA14450.023.73194510
GTACAAT13950.023.58146326
GTGATAG19450.023.2162653
ACTCTAG7200.022.8500735
GGTAACA14550.022.8466813
GAGGTAA16000.021.43249911
AACTAGA15250.021.11044119
CTAACCT7300.021.0954642
CGGGGAG8700.020.524971
TAGAGGT17500.020.3974119
ACAACTA17300.020.22649217
CGTGATA23800.019.857342
GGGGGAA50850.019.41451867
GGGGAAA48600.019.37709668
Produced by FastQC (version 0.11.2)