FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432278
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG496411.1199884122791937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT10800.041.8095936
ACTCTAG11300.040.2702875
TCTAGTT11600.039.8309827
CTAGTTG13350.035.6524358
GTACTCT13400.034.2216573
TACTCTA16200.030.4665954
GGGGGAA73900.026.29013667
GGGGAAA73450.025.92694968
TGTTACC17250.025.3550413
TGTACTC18650.024.7778662
ACCTCTA17750.024.6410917
TACCTCT17650.024.58245516
TAGTTGT21100.023.5525239
GGGGGGA91500.022.4194966
GGGAAAA87350.022.20320369
TTACCTC19550.022.19336715
TGGACAC21050.020.94741428
GACACCT21000.020.8351130
GGAAAAA100300.020.17417170
CTCTAAT22100.019.79226119
Produced by FastQC (version 0.11.2)