FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3848064
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG843702.192531101353824No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC86090.2237228902637794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66020.17156679306789074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT15250.046.5780121
CCAATAG15800.042.5299538
AACTAGA16900.041.8234119
AGAGGTA18000.041.79139310
ACTAGAG17000.041.7832220
CAATAGA16400.041.1873839
CAACTAG17500.040.98930418
AGAGTAC19000.037.01954723
TAGAGTA19250.036.90234422
TCGCTTA15100.035.9308652
GGTAACA20750.035.91367313
TAGAGGT21100.035.8223659
AGGTAAC21200.035.6473712
GAGGTAA21200.035.48325711
GTACAAT20400.035.3380526
ATTAGAG21850.034.601767
ACAACTA22400.032.80010617
CCTACTA7950.032.1433534
ATAGAAT21300.032.04180541
TAGAATC21150.031.60712442
Produced by FastQC (version 0.11.2)