FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220293
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314211.4151735829460346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG37670.1696622923190768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG24510.11039083580410333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG4600.044.8880438
GTGGTCG4900.041.43115654
TCGGTGG4550.040.7699451
CGGTGGT4900.040.00249552
GGTCGCC5300.038.96294456
CCAATAG3250.035.53587738
GGTGGTC6100.034.4283853
TAGATCT5600.034.3763745
GTAGGAT6450.034.1904932
GGAAAAA111250.033.07692370
GGGGAAA105900.032.23379568
ATAGGGT6300.032.2198837
GGGAAAA110000.031.86131969
GAGTGTA6400.031.7128728
GGGGGAA107950.031.2973467
TGGTCGC6500.031.23131455
GATAGGG6400.031.16820336
AGGGTGT6500.030.69007739
AGATCTC6050.030.6623846
CTAGAGT4000.030.61794321
Produced by FastQC (version 0.11.2)