FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027105
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG779301.9351370277159399No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG55960.13895838325546514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT9100.035.3881536
GTACTCT9450.034.8178673
TACTCTA10700.033.040284
TCTAGTT10450.032.155537
ACTCTAG10250.031.076285
CTAGTTG10400.030.2858498
GGGGAAA111400.028.75313268
GGGAAAA113550.028.51700269
GGGGGAA112900.028.46413667
GGAAAAA122150.027.36901570
TACCTCT11500.027.07991416
TGTTACC12000.025.65967413
ACCTCTA12450.025.01357317
TAGTTGT14900.022.313519
TGTACTC15150.021.9507542
GGGGGGA155100.021.64492666
TGGACAC14550.020.4442128
GTTACCT15600.020.18680814
TTACCTC16600.019.81387715
GACACCT15700.019.172730
Produced by FastQC (version 0.11.2)