FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4928912
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2133294.3281154137059055No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC134250.27237248301450706No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATC69540.14108590293354803Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG67320.13658186634291705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG59240.12018879622926926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT21500.051.75614521
CCAATAG22450.048.79806538
ACTAGAG24300.046.2244820
AGAGGTA26000.045.35456510
AACTAGA25150.043.82696519
TAGAGGT27150.043.4396369
TAGATCT13400.042.31621645
CAACTAG26300.041.77752718
ATTAGAG28950.040.9850737
CAATAGA27100.040.4249639
GGTGGTC14650.039.9014553
CGGTGGT14750.039.39362352
GGTAACA29500.039.37883813
TAGAATC27650.039.36927842
GAGGTAA30400.038.78969211
TAGAGTA28900.038.62713622
TCGGTGG15250.037.87211251
AGAGTAC29650.037.2963623
GTACAAT30050.037.2672426
GTGGTCG15900.037.20483854
Produced by FastQC (version 0.11.2)