FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_vgt0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_vgt0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90220.22557075250923087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT3450.022.3250891
GGCCTAC1205.7099183E-520.4223841
AGTCTAG2402.3646862E-1120.41293714
CTAGGCC950.00720461218.42505668
CTAGGTA4950.015.5581592
GTCTAGG3454.0199666E-914.20030315
TGGCAAC13650.014.1012219
CTAGGCT4102.2555469E-1013.65604117
CTACTAG4659.094947E-1213.549129534
TCTAGGC4152.728484E-1013.4915116
TGGGAGT5500.013.3658732
TATCGAC13050.013.1424845
TTACTCG14150.013.1094442
AGTATAC11300.013.009562531
GAACTAC8150.012.4548263
GGGGGGA19850.012.16889366
GTAGTAT7700.011.81602313
GGCAACT15750.011.77562610
GAGTATA11900.011.76535130
TACTCGT16200.011.66657743
Produced by FastQC (version 0.11.2)