FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_sigg45_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_sigg45_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398164
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6146815.437859776373555No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC59521.494861413889754Illumina Single End PCR Primer 1 (96% over 33bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44681.122150671582564No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC44091.1073326568951487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33890.8511568097567836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG23230.5834279342180609No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG22340.561075335791282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG21620.5429923348168091No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG21140.5309370008338272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG15620.39230066002953556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG12620.3169548226358988No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT11340.2848072653479471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG9990.25090163852081054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG9150.2298048040505922No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT8270.2077033584151254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG7560.18987151023196472No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG6700.16827237017912217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG6230.1564681889874524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG6090.1529520499090827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG5790.145417466169719No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG5180.13009714589967955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG4670.11728835354276128No Hit
CCTTGTAAGCGATGTGATTCTATTGGAAAAAATTGTACTCTAGTTGTTAC4340.10900031142946122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC8000.060.38096261
GGTCGCC8650.058.67631556
CGTATCA8400.057.92238262
CGCCGTA8800.057.67614759
GTGGTCG8650.057.0576654
TCGGTGG7800.056.992851
GTCGCCG8850.056.95477357
CTCGGTG7600.056.65032650
TCTCGGT7600.056.18975449
GTATCAT8350.056.17320663
TAGATCT8400.055.83884445
GTAGATC8600.055.7613244
TGGTCGC8850.055.37269655
GGTGGTC8550.055.26861653
TCGCCGT9100.055.0054358
CGGTGGT8300.054.82468852
AGATCTC8400.054.58872246
ATCATTA8700.054.3225365
GTGTAGA9400.053.62231442
ATCTCGG8300.053.559548
Produced by FastQC (version 0.11.2)