FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_sige45_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_sige45_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571536
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96371.6861580022955684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG1900.038.685615
CTCTAGT2050.034.147586
TGTACTC2100.033.3403782
CTAGTTG2250.031.1095168
TACCTCT2350.029.7857116
TCTAGTT2400.029.1677237
TAGTTGT2650.029.0551159
ACCTCTA2450.028.56996517
GCCTAGC500.00720303128.00346670
CCGTGGG500.007209187427.99856621
GGGGAAA13700.027.3391568
GGGGGAA14700.026.9080967
TTAGAGT1606.548362E-1126.24865712
GTTACCT2900.024.13669414
TGTTACC2900.024.13669413
GGGAAAA15600.023.78499269
GTGATAG1802.8558134E-1023.3341793
GGTAGCG901.9267488E-423.3341797
CTCTAAT3000.023.33213819
GTTGTTA3050.022.94964411
Produced by FastQC (version 0.11.2)