FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_sige35_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_sige35_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041834
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1719382.8457915262153843No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG185480.3069928766662573No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC116650.19307051468146924No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA110120.18226253816308094No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG108640.17981295083578924No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA100930.1670519249618576No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC96270.1593390351340338No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC85200.14101678397652104No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT73300.12132077776383793No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG73230.12120491890376332No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT63520.10513363988484291No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT63470.10505088355621818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT9000.035.389766
TCTAGTT10450.031.1478027
GGGGGAA217300.028.07919967
GGGGAAA212350.027.77782668
ACTCTAG11600.027.7595335
GGAAAAA222050.027.13283370
TTAGAGT15300.025.1567312
TACAAGG53450.025.1410338
GGCGTGA33250.024.2181381
ACCTTAG17000.023.4668929
CCTTAGA16550.023.2570610
GTGATAG37650.023.2411374
CTAGTTG13900.023.161828
TAGTTGT14450.023.0067589
GGGAAAA257450.022.9532769
CTTAGAG17200.022.78503411
TGTACTC13850.022.7474442
GGGGGGA297400.021.94095866
TACCTCT14650.021.73485416
ACAAGGA62550.021.4834279
Produced by FastQC (version 0.11.2)