FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_s87745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_s87745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497328
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142330.5699291402651153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA22150.020.3908467
ACTCTAG3000.019.8362625
GGGGGGA27750.017.66380366
TCTAGTT4250.017.2956317
CTAGTTG3850.017.2697438
GGGGAAA27700.016.68450568
GTCGACT3108.785719E-1015.8129031
CTCTAGT4100.015.3681236
TAGGTCC1708.179377E-414.41187347
CCTACTA2707.4392483E-714.26222234
GGGAAAA34550.013.68086669
TAGTACG1800.00126125813.60930424
ACCTACT3354.1556632E-813.58491133
GTACCAC1850.001547386413.2451985
TGGTAGT1850.001553233813.23856915
GTGCTAG2153.244593E-413.02022422
ACCTCTA4601.0186341E-1012.93021717
GGGGGTA3254.223839E-712.92757566
GACACCT6600.012.72940730
GGCTAGA1950.002301451512.56419651
Produced by FastQC (version 0.11.2)