FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780873
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1534224.057845899611015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50100.1325090792523314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG39690.10497575559930206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAA193450.032.50072570
GGGAAAA192850.031.33117169
TCGGTGG6600.031.28962351
GGGGAAA192950.030.9331168
GGGGGAA202550.029.7089767
AGTGTAC7800.028.71916829
GGTGGTC7250.028.48472253
GGTCGCC7900.027.0257556
CGGTGGT7850.026.30754752
TAGATCT7500.023.80069745
GGGGGGA265950.023.58750366
GTGGTCG8850.022.9397454
GAGTGTA10150.022.41148628
GTAGATC8300.022.35005244
GTCCTGG8750.021.59806336
TGGTCGC10700.021.26230255
GTAGGGA11250.021.15253819
GTCGTGT11600.021.11683814
AGAGTGT11050.020.58611727
TCCTGGT9700.020.56625437
Produced by FastQC (version 0.11.2)