FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3021358
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2158907.145462404653802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG125100.41405222419852267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG51880.1717108664382043No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC43200.14298206303258337Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG39320.1301401555194717No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA36710.1215016558779198No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG34170.11309484013480031No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT32670.10813018516839118No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC32490.10753442657242206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG31340.10372819109817506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG30410.1006501050190014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG8900.048.76523251
CGGTGGT9000.048.61390752
GGTGGTC9250.046.54321753
GTAGATC10300.043.49836344
TAGATCT10700.040.89087745
GTGGTCG10800.040.5115954
GGTCGCC11250.039.82253356
GTGTAGA11950.038.6613942
AGTGTCT11650.038.15796329
TGTGTAG11900.037.64735441
GGAAAAA259600.037.61108870
GAGTGTC12400.036.6883728
ACTAGAG6800.036.53593420
CGTGTAG13050.036.46632816
CTCGGTG12000.036.1675550
AGAGTGT13150.035.66036227
TGGTCGC13150.035.13393455
TAGGGAA13500.034.99216520
AGAGTAC7100.034.5010323
GTGTCTT13100.034.468230