FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256615
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG614384.889166530719432No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG56040.4459599797869674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG15080.12000493388985489No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA13620.10838641907028007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG4600.047.1784975
GTACTCT5000.043.4042173
TGTACTC5150.042.1400222
TACTCTA5550.041.625674
CTCTAGT5400.039.5408826
GGAAAAA82750.035.5772970
TTGTACT6250.034.170121
TCTAGTT6500.033.3838777
GGGAAAA88550.031.94240869
GGGGAAA91600.030.07508368
GGGGGAA93300.029.45205367
TGTTACC7500.028.92463513
TAGTTGT7500.027.997159
ACCTCTA7800.027.81214517
CTAGTTG7450.027.7152988
TTACCTC8200.026.88215815
TACCTCT8300.026.13671716
GTTACCT8500.025.11009414
CTCTAAT8650.023.86565419
TTGTTAC9350.023.20157612
Produced by FastQC (version 0.11.2)