FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864892
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG615711.5930846191821144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68270.17664141714697332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA83050.027.56704767
GGGGAAA83100.027.46620668
GGGAAAA97000.023.63859669
GGGGGGA104450.023.12558666
GGAAAAA113000.021.15896270
GGGGTAA8900.016.91338567
GGGGGTA9700.016.6011566
GATTCGA13350.014.9455524
GGGGTCT3456.1061655E-813.1921541
TAGACTC8550.012.69005253
ATTCGAC16150.012.5711165
CGGCCTA6450.012.48061448
ACTGCGC8450.012.00980811
GGTGGTC5054.802132E-1011.78217253
GGATTCG18800.011.73008253
AACTAAC24050.011.49687563
GCTAGAT3104.790786E-511.2935311
AGACTCC9300.011.29031754
ATCCGAG9700.011.18773368
ACTAACA25050.011.17763864
Produced by FastQC (version 0.11.2)