FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723773
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48330.2803733438219534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT1553.4984623E-822.5885561
CTATAAC3900.022.43204111
CGTAGTA2108.367351E-1121.66294112
GGTACCT850.00379402520.5858921
CTAACGT4200.020.0070061
GGGGGGA9800.017.50308266
GGGTAAT2651.875378E-917.1758271
AACTATA5600.016.872599
GTAGTAT3108.8584784E-1015.80373313
ACCCGCG5550.015.76671344
GTCTAAC2704.382673E-815.5610051
CTAGGTA2251.7603506E-615.5587462
GTGCAAC1350.002947933715.5551366
GGGGGAA10000.015.40271367
CGTAGGC1859.0405825E-515.13472935
ACTATAA6350.014.8793310
TCTAACG3101.4983016E-814.6804312
CCTACTA1708.1814907E-414.41095931
GACACCC6150.014.22808441
CGCGTCT3707.003109E-1014.194161
Produced by FastQC (version 0.11.2)