FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649405
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1390573.8104019696361466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG121190.33208153109890515No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA46020.12610274825622259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38200.10467459763988925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG12600.048.89385
CTCTAGT13700.045.4790236
GTACTCT13950.044.162143
TGTACTC14600.042.2018052
TCTAGTT16700.037.5171937
CTAGTTG18050.033.928568
TTGTACT18300.033.673351
TACTCTA19400.033.3796424
GGAAAAA203350.032.6755770
TAGTTGT19250.031.9953219
GGGAAAA202000.031.33417369
GGGGAAA205350.030.27704668
ACCTCTA19750.029.94293817
TGTTACC19900.029.71723713
TACCTCT19950.029.46735816
GGGGGAA209850.029.427667
TTACCTC21500.027.66847615
GTTACCT21750.027.02867714
GTTGTTA25900.023.77867711
CACCTAC8800.023.467332
Produced by FastQC (version 0.11.2)