FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712958
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149400.2615107620255566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96550.1690017675606927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA122050.026.99103467
GGGGAAA123450.026.76977768
GGGAAAA139200.024.32036669
GGGGGGA151150.022.25783566
GGAAAAA174600.020.2516370
AGTCTAG2104.2920874E-818.32919314
TAGTGTA5700.016.5788340
AGTGTAG5100.016.47047241
GGGACTA2251.762608E-615.5583072
AACTATA10400.015.4790359
CTATAAC10650.014.12851111
GTGGTCG6600.013.78886854
GGTGGTC6650.013.68483453
GGGGTAA15900.013.43075367
GGGTTTT57300.013.31462336
TTGTGGG25350.012.97866745
GTGGGTT17650.012.89010634
GGGGTTT53700.012.77525535
ACTATAA12500.012.597486510
TGTGTGG17700.012.45797646
Produced by FastQC (version 0.11.2)