FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175335
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97260.4471035495682274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT2155.456968E-1222.7961481
ACCCACG1506.4014966E-720.99633212
GTCGACT850.003786587920.5931591
GGGTAAT4050.019.8812961
GGGGGGA15400.019.0937266
CTAGGTA2607.2759576E-1118.849362
GGGATCC3800.017.50257766
TGGGAGT2151.0965341E-616.281842
TAGGAGT2151.1004904E-616.27622614
CCACGTA1300.002289079116.1565932
GGGGGAA15700.016.05331867
GGGGATC4350.015.28960565
CGTAGTA2101.5854577E-514.9973812
ACACGAC8200.014.94019253
AACCCAC2403.455958E-614.58145711
TCAGTCG8800.014.316339510
GGGGAAA16300.014.17386568
AGGTAAG3758.60382E-1014.0001284
GGATCCG4555.456968E-1213.84819167
AGTGTAG1550.00737450713.54913456
Produced by FastQC (version 0.11.2)