FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_oat0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_oat0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562640
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1027096.572787078277786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG88450.5660292837761737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG23630.1512184508268059No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG19750.1263886755746685No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG16620.10635847028106281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA3350.037.6144184
GGAAAAA128100.036.5394870
GTACTCT3600.035.0023083
TCTAGTT3750.034.5345047
GGGAAAA144100.031.58344369
CTCTAGT3900.031.4123276
GGGGAAA148250.029.66026168
GGGGGAA152200.028.84449667
ACTCTAG4250.028.8254285
TGTACTC4700.026.0697172
GGTGGTC1951.8189894E-1225.12905753
GAAAAAA80650.024.30881170
GGGGGGA206550.021.76305666
TAGTTGT5850.021.5350559
CCGTGTA3100.021.45165340
TACCTCT5250.021.32859416
GTGTAGA2800.021.2500442
CTAGTTG6000.020.9966778
GGGGTAA9900.019.80308767
CTAGTGG1601.186585E-619.68816656
Produced by FastQC (version 0.11.2)