FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_csfg35_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_csfg35_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944719
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG684081.1507356361166945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA112300.026.8700767
GGGGAAA122050.024.40804968
CTCTAGT7600.021.1856446
GGGGGGA144950.021.15569166
ACTCTAG7250.020.7602025
CGGTGGT6750.020.74197252
GGGAAAA155200.019.51152869
TTAGAGT11450.018.94814912
TCTAGTT8950.018.7722237
CTAGTTG8250.018.6654198
CCTTAGA12200.018.35788510
GGAAAAA176550.018.08397970
TCGGTGG7850.017.3889851
ACCTTAG13000.016.9604059
CTTAGAG13150.016.76510811
GGTGGTC9150.016.06652853
GTGATAG17900.015.0568883
GTAGATC10500.015.00051144
GTAGGGA11350.014.49108319
CTAGATT12100.014.17391957
Produced by FastQC (version 0.11.2)