FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t45_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t45_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854251
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG508921.3204121890349123No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG39190.10167993729520988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG5500.035.0024645
TCTAGTT7000.030.0009467
CTCTAGT6600.029.168726
CTAGTTG6950.028.7018438
TAGTTGT7850.027.6402389
GTACCAC2150.026.0483475
GGGGGAA73850.024.64948567
TCGGTGG4750.024.31655551
CCTTAGA6500.024.22650310
GGGGAAA77050.023.21684868
ACCTTAG7300.022.531749
TTAGAGT6700.022.45785712
ACCTCTA9550.021.98451617
CGGTGGT5550.021.4432352
GTGTAGA6600.021.21223842
CTTAGAG7900.019.93294111
ACCTACT6000.019.83421533
GGGAAAA94500.019.18977469
TACCTCT10100.019.0553116
TGTACTC10300.019.0329382
Produced by FastQC (version 0.11.2)