FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n02_79t35_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79t35_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794795
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG801131.6708326424800226No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG181790.3791402969261459No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA122030.2545051456840178No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG106860.22286667104641594No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC104210.2173398445606121No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA91800.19145761184784751No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC77330.16127905364045805No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC67720.14123648664854285No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC62300.12993256228889868No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT57470.11985913892043351No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG51650.10772097660066801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT12850.040.040276
ACTCTAG13300.039.2118575
TCTAGTT14050.036.8691947
TGTACTC15000.035.940552
CTAGTTG17100.030.0846618
TAGTTGT18800.028.1088459
GGGGGAA134900.027.61031767
GGGGAAA139500.026.6998768
GGAAAAA164150.024.52518370
TGTTACC20950.024.05307813
TTGTACT23050.023.695361
GGCGTGA26550.023.6046831
ACCTCTA21500.022.62395717
TACCTCT21950.022.16014116
TTACCTC22700.021.89045115
GTGATAG30750.021.6269763
GTTGTTA24800.021.30676311
GTTACCT23200.021.26783814
GGGGGGA178950.021.1072466
GGGAAAA182450.020.81810269
Produced by FastQC (version 0.11.2)